The polyhydroxyalkanoate metabolism controls carbon and energy spillage in Pseudomonas putida
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چکیده
منابع مشابه
Controlled autolysis facilitates the polyhydroxyalkanoate recovery in Pseudomonas putida KT2440
The development of efficient recovery processes is essential to reduce the cost of polyhydroxyalkanoates (PHAs) production. In this work, a programmed self-disruptive Pseudomonas putida BXHL strain, derived from the prototype medium-chain-length PHA producer bacterium P. putida KT2440, was constructed as a proof of concept for exploring the possibility to control and facilitate the release of P...
متن کاملMonitoring differences in gene expression levels and polyhydroxyalkanoate (PHA) production in Pseudomonas putida KT2440 grown on different carbon sources.
Pseudomonas putida has a variety of potential uses in bioremediation and biosynthesis of biodegradable plastics. P. putida is able to utilize a wide range of carbon sources. In this study, P. putida KT2440 was grown on glucose, glycerol, citrate, or fatty acid (lauric acid) as the sole carbon source. Differences in expression levels of genes involved in the Entner-Doudoroff pathway, glycerol me...
متن کاملFunctional metagenomics using Pseudomonas putida expands the known diversity of polyhydroxyalkanoate synthases and enables the production of novel polyhydroxyalkanoate copolymers
Functional metagenomics using Pseudomonas putida expands the known diversity of 1 polyhydroxyalkanoate synthases and enables the production of novel polyhydroxyalkanoate 2 copolymers 3 4 14 15. CC-BY 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not. Abstract 17 Bacterially produced biodegradable pol...
متن کاملThe functional structure of central carbon metabolism in Pseudomonas putida KT2440.
What defines central carbon metabolism? The classic textbook scheme of central metabolism includes the Embden-Meyerhof-Parnas (EMP) pathway of glycolysis, the pentose phosphate pathway, and the citric acid cycle. The prevalence of this definition of central metabolism is, however, equivocal without experimental validation. We address this issue using a general experimental approach that combine...
متن کاملRegulatory Tasks of the Phosphoenolpyruvate-Phosphotransferase System of Pseudomonas putida in Central Carbon Metabolism
UNLABELLED Two branches of the phosphoenolpyruvate-phosphotransferase system (PTS) operate in the soil bacterium Pseudomonas putida KT2440. One branch encompasses a complete set of enzymes for fructose intake (PTS(Fru)), while the other (N-related PTS, or PTS(Ntr)) controls various cellular functions unrelated to the transport of carbohydrates. The potential of these two systems for regulating ...
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ژورنال
عنوان ژورنال: Environmental Microbiology
سال: 2012
ISSN: 1462-2912
DOI: 10.1111/j.1462-2920.2011.02684.x