The Post-genomic Era of Trichoderma reesei : What's Next?

The Post-Genomic Era for Cotton

Background and Rationale The scientific infrastructure in support of cotton research and improvement took a ‘giant leap forward’ with the release of the first ‘gold-standard’ cotton reference genome sequence on 5 January 2012. Later in 2012, two independent publications (Paterson et al. 2012; Wang et al. 2012) provided initial descriptions of the basic genome of cotton, with one of these also r...

Crystallography in the post-genomic era

X-ray crystallography is the most powerful technique to determine the three-dimensional structure of proteins. Several steps are required to solve the structure of proteins including cloning and expression of the gene, protein purification, crystallization, X-rays diffraction, data collection and structure determination. The vast amount of data generated by genomic studies has accelerated the d...

Novel Strategies for Genomic Manipulation of Trichoderma reesei with the Purpose of Strain Engineering

The state-of-the-art procedure for gene insertions into Trichoderma reesei is a cotransformation of two plasmids, one bearing the gene of interest and the other a marker gene. This procedure yields up to 80% transformation efficiency, but both the number of integrated copies and the loci of insertion are unpredictable. This can lead to tremendous pleiotropic effects. This study describes the de...

Bioinformatics in the post-genomic era.

Recent years saw a dramatic increase in genomic and proteomic data in public archives. Now with the complete genome sequences of human and other species in hand, detailed analyses of the genome sequences will undoubtedly improve our understanding of biological systems and at the same time require sophisticated bioinformatic tools. Here we review what computational challenges are ahead and what ...

Ophthalmology in the post-genomic era.