The utility of the PCR melting profile technique for typing Corynebacterium diphtheriae isolates
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چکیده
منابع مشابه
Use of amplified fragment length polymorphisms for typing Corynebacterium diphtheriae.
Amplified fragment length polymorphism (AFLP) was investigated for the differentiation of Corynebacterium diphtheriae isolates. Analysis using Taxotron revealed 10 distinct AFLP profiles among 57 isolates. Strains with ribotype patterns D1, D4, and D12 could not be distinguished; however, the technique discriminated isolates of ribotype patterns D3, D6, and D7 further. AFLP was rapid, fairly in...
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A throat swab from a 9 year old girl with pharyngitis yielded a non-toxigenic strain of Corynebacterium diphtheriae var mitis and Streptococcus group G. C pseudodiphtheriticum was isolated from the throats of two of her four brothers. In each case the isolate was sent to the reference laboratory before full identification. The growth was found to be mixed for one brother; the other isolate bein...
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Antibiotic susceptibility patterns of recent isolates of Corynebacterium diphtheriae.
A variety of factors which might affect zone sizes were studied with strains of Corynebacterium diphtheriae; a standard disc method for antimicrobial sensitivity testing was used. Moderate variations in inoculum size, inoculum preparation, and pH of Mueller Hinton agar (MHA) did not appreciably affect zone sizes. The addition of blood to MHA was necessary to insure the growth of all C. diphther...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2014
ISSN: 0266-8254
DOI: 10.1111/lam.12274