Time-course transcriptome analysis of host cell response to poxvirus infection using a dual long-read sequencing approach

Abstract Objective In this study, we applied two long-read sequencing (LRS) approaches, including single-molecule real-time and nanopore-based methods to investigate the time-lapse transcriptome patterns of host gene expression as a response Vaccinia virus infection. Transcriptomes determined using short-read approaches are incomplete because these platforms inefficient or fail distinguish between polycistronic RNAs, transcript isoforms, transcriptional start sites, well readthroughs overlaps. Long-read is able read full-length nucleic acids can therefore be used assemble complete atlases. Results work, identified number novel transcripts isoforms Chlorocebus sabaeus. Additionally, analysis most abundant 768 revealed significant overrepresentation class genes in “regulation signaling receptor activity” Gene Ontology annotation result viral