Time-Course Transcriptome Profiling of a Poxvirus Using Long-Read Full-Length Assay

Viral transcriptomes that are determined using first- and second-generation sequencing techniques incomplete. Due to the short read length, these methods inefficient or fail distinguish between transcript isoforms, polycistronic RNAs, transcriptional overlaps readthroughs. Additionally, approaches insensitive for identification of splice start sites (TSSs) and, in most cases, end (TESs), especially isoforms with varying ends, multi-spliced transcripts. Long-read is able full-length nucleic acids can therefore be used assemble complete transcriptome atlases. Although vaccinia virus (VACV) does not produce spliced its has a high diversity TSSs TESs, degree polycistronism leads enormous complexity. We applied single-molecule, real-time, nanopore-based investigate time-lapse patterns VACV gene expression.