Tolerance to challenges miming gastrointestinal transit by spores and vegetative cells of Bacillus clausii
نویسندگان
چکیده
منابع مشابه
Tolerance to challenges miming gastrointestinal transit by spores and vegetative cells of Bacillus clausii.
AIMS To study Bacillus clausii from a pharmaceutical product (Enterogermina O/C, N/R, SIN, T) and reference strains (B. clausii and Bacillus subtilis) for eco-physiological aspects regarding the gut environment. METHODS AND RESULTS Spores and vegetative cells were challenged in vitro miming the injury of gastrointestinal transit: pH variations, exposure to conjugated and free bile salts, micr...
متن کاملInactivation of Bacillus cereus vegetative cells by gastric acid and bile during in vitro gastrointestinal transit
UNLABELLED BACKGROUND The foodborne pathogen Bacillus cereus can cause diarrhoeal food poisoning by production of enterotoxins in the small intestine. The prerequisite for diarrhoeal disease is thus survival during gastrointestinal passage. METHODS Vegetative cells of 3 different B. cereus strains were cultivated in a real composite food matrix, lasagne verde, and their survival during sub...
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A preparation of Bacillus anthracis (Sterne strain) spores was used to evaluate commercially available reagents and portable equipment for detecting anthrax contamination by using real-time PCR and was used to assess the fate of spores added directly to bulk tank milk. The Ruggedized Advanced Pathogen Identification Device (RAPID) was employed to detect spores in raw milk down to a concentratio...
متن کاملExtraction and detection of DNA from Bacillus anthracis spores and the vegetative cells within 1 min.
The use of a combination of low-cost technologies to both extract and detect anthrax DNA from spores and vegetative cells in two steps within 1 min is described. In a cavity, microwave energy is highly focused using thin-film aluminum "bow-tie" structures, to extract DNA from whole spores within 20 s. The detection of the released DNA, from less than 1000 vegetative cells, without additional pr...
متن کاملProperties of Fructose 1,6-Diphosphate Aldolases from Spores and Vegetative Cells of Bacillus cereus1
Fructose 1,6-diphosphate aldolase from cells of Bacillus cereus appears to be typical Class II aldolase as judged by its functional and physical properties: Spore and vegetative cell aldolase had similar enzymatic, immunochemical, and heat resistance properties in the absence of calcium, but they differed in their thermal stabilities in the presence of calcium, their Stokes' radii, their mobili...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2006
ISSN: 1364-5072,1365-2672
DOI: 10.1111/j.1365-2672.2006.03042.x