Toward a Semisynthetic Stress Response System To Engineer Microbial Solvent Tolerance

Toward a Semisynthetic Stress Response System To Engineer Microbial Solvent Tolerance

UNLABELLED Strain tolerance to toxic metabolites is an important trait for many biotechnological applications, such as the production of solvents as biofuels or commodity chemicals. Engineering a complex cellular phenotype, such as solvent tolerance, requires the coordinated and tuned expression of several genes. Using combinations of heat shock proteins (HSPs), we engineered a semisynthetic st...

Microbial mechanisms of tolerance to weak acid stress

Carboxylic acids are ubiquitous molecules found in microbial metabolic pathways and that have been explored for a wide array of applications including food preservation (e.g., acetic, propionic, benzoic, and sorbic acids), chemotherapy (e.g., the analgesic acetylsalicylic acid, the immunosuppressor mycophenolic acid or the antimalarial drugs artesunic and artemisinic acids) or agriculture (e.g....

Engineering Sugar Utilization and Microbial Tolerance toward Lignocellulose Conversion

Production of fuels and chemicals through a fermentation-based manufacturing process that uses renewable feedstock such as lignocellulosic biomass is a desirable alternative to petrochemicals. Although it is still in its infancy, synthetic biology offers great potential to overcome the challenges associated with lignocellulose conversion. In this review, we will summarize the identification and...

Trump’s Zero-tolerance Policy: Would a Political Response to a Humanitarian Crisis Work?

Toward a Smart Distribution System Expansion Planning by Considering Demand Response Resources

This paper presents a novel concept of "smart distribution system expansion planning (SDEP)" which expands the concept of demand response programs to be dealt with the long term horizon time. The proposed framework, integrates demand response resources (DRRs) as virtual distributed generation (VDG) resources into the distribution expansion planning. The main aim of this paper is to develop and ...