Towards Development, Maintenance, and Standardized Phenotypic Characterization of Single?Seed?Descent Genetic Resources for Lupins

Well-characterized genetic resources are fundamental to maintain and provide the various genotypes for pre-breeding programs production of new cultivars (e.g., wild relatives, unimproved material, landraces). The aim current article is protocols characterization two lupin crop species: European Lupinus albus American mutabilis. Intelligent nested collections lupins derived from homozygous lines (single-seed descent) being developed, established, exploited using cutting-edge approaches genotyping, phenotyping, data management, analysis within INCREASE project (EU Horizon 2020). This will allow us predict phenotypic performance genotyped lines, further boost research development in lupins. Lupins stand out due their high-quality seed protein (?40% dry weight) other primary components seeds, which include fatty acids, dietary fiber, minerals. potential as a highlighted by multiple benefits plant-based food terms security, nutrition, human health, sustainable production. use foods, along with well-studied widely used legumes, also greatly diversified palette meet Global Goals Sustainable Development improve people's lives 2030. © 2021 Authors. Current Protocols published Wiley Periodicals LLC. Basic Protocol 1: Lupin descriptors 2: imaging 3: Standardized grown towards increase (development single-seed descent resources)