Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili
نویسندگان
چکیده
منابع مشابه
The role of mannose sensitive and mannose resistant pili of Escherichia coli in urinary tract infection
In order to study the relationship between hrmolysin / ashesins of Escherichia coli and occurrence of urinary tract infection ( UTI), the capacity of 570 isolated E.coli in agglutination of human or guinea pig erythrocytes and their lysis were tested. These isolates were obtained from the urine of patients with bacteriuria and from the stool of healthy people. Three patterns of hemagglutinat...
متن کاملGenes for CS2 pili of enterotoxigenic Escherichia coli and their interchangeability with those for CS1 pili.
We have cloned and sequenced the DNA needed for production of CS2 pili in Escherichia coli K-12. The four open reading frames, cotB, cotA, cotC, and cotD, show homology with the genes needed for production of CS1 and CFA/I pili, which are also found on enterotoxigenic E. coli associated with human diarrheal disease. We also report that CotA plus CotB interact with the CS1 gene products CooC and...
متن کاملMechanism of Sperm Immobilization by Escherichia coli
AIM To explore the influence of Escherichia coli on the motility of human spermatozoa and its possible mechanism. METHODS Highly motile preparations of spermatozoa from normozoospermic patients were coincubated with Escherichia coli for 4 hours. At 1, 2 and 4 hours of incubation, sperm motility was determined. The factor responsible for sperm immobilization without agglutination was isolated ...
متن کاملThe Ability of Cellulose Polysaccharide and Curli Pili Production in Uropathogenic Escherichia Coli and its Association with Biofilm Formation Intensity
Abstract Background and Objective: the Formation of urinary infection by uropathogenic E.coli needs numerous virulence factors and biofilm formation is among these factors. Bacteria that form biofilms express phenotype traits that appear according to the bacteria type. Cellulose is an important compound on the outside of E.coli causing bacte...
متن کاملHeterologous Bacillus subtilis-Escherichia coli Gene Fusion
Translational coupling was demonstrated in a gene fusion in which the promoter and the N-terminal region of the Bacillus subtilis subtilisin (aprA) gene were fused to a promoterless Tn9-derived chloramphenicol acetyltransferase (CAT; EC 2.3.1.28) gene. Expression of this gene fusion results in the production of a native-sized CAT product, whereas the Tn9-derived CAT gene is usually not translat...
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ژورنال
عنوان ژورنال: Microbial Biotechnology
سال: 2018
ISSN: 1751-7915,1751-7915
DOI: 10.1111/1751-7915.13309