Towards quantitative mass spectrometry-based metabolomics in microbial and mammalian systems
نویسندگان
چکیده
منابع مشابه
Mass spectrometry-based metabolomics.
This review presents an overview of the dynamically developing field of mass spectrometry-based metabolomics. Metabolomics aims at the comprehensive and quantitative analysis of wide arrays of metabolites in biological samples. These numerous analytes have very diverse physico-chemical properties and occur at different abundance levels. Consequently, comprehensive metabolomics investigations ar...
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An analytical method was set up suitable for the analysis of microbial metabolomes, consisting of an oximation and silylation derivatization reaction and subsequent analysis by gas chromatography coupled to mass spectrometry. Microbial matrixes contain many compounds that potentially interfere with either the derivatization procedure or analysis, such as high concentrations of salts, complex me...
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untargeted. Targeted metabolomics focuses on a subset of metabolites involved in one or more pathways; the chemical identities of metabolites to be measured are known, authentic standards are available in these studies. The goal of untargeted metabolomics is to observe global metabolite profiling differences between sample types, experiments are designed to maximise the coverage of metabolites....
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Metabolomics is a rapidly expanding field with applications in areas such as medicine, agriculture, or food safety. Tandem mass spectrometry (MS) is one of the main technologies that drives the field forward. Optionally coupled to a chromatographic element, MS can capture detailed snapshots of an organism’s metabolome. The resulting data sets are complex and difficult to analyse due to the mult...
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Breast cancer accounts for the largest number of newly diagnosed cases in female cancer patients. Although mammography is a powerful screening tool, about 20% of breast cancer cases cannot be detected by this method. New diagnostic biomarkers for breast cancer are necessary. Here, we used a mass spectrometry-based quantitative metabolomics method to analyze plasma samples from 55 breast cancer ...
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ژورنال
عنوان ژورنال: Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences
سال: 2016
ISSN: 1364-503X,1471-2962
DOI: 10.1098/rsta.2015.0363