Training Course on Somatic Genotoxicity Assays with Drosophila: Somatic Mutation and Recombination Tests (SMART)

Genotoxicity testing of some metals in the Drosophila wing somatic mutation and recombination test.

High-Throughput In Vivo Genotoxicity Testing: An Automated Readout System for the Somatic Mutation and Recombination Test (SMART)

Genotoxicity testing is an important component of toxicity assessment. As illustrated by the European registration, evaluation, authorization, and restriction of chemicals (REACH) directive, it concerns all the chemicals used in industry. The commonly used in vivo mammalian tests appear to be ill adapted to tackle the large compound sets involved, due to throughput, cost, and ethical issues. Th...

Genotoxicity testing of Cecropia obtusifolia extracts in two in vivo assays: the wing somatic mutation and recombination test of Drosophila and the human cytokinesis-block micronucleus test.

Cecropia obtusifolia Bertol. (Cecropiaceae) is a tree that grows in secondary vegetation in the tropical rain forest along both coasts of Mexico. Its leaves are used in folk medicine for the treatment of diabetes mellitus type 2. The aim of the present studies was the evaluation of possible genotoxic effects of the aqueous extract from the leaves of Cecropia obtusifolia by means of two differen...

Interchromosomal Effects on Somatic Recombination in Drosophila Melanogaster.

RECOMBINATION in somatic cells of Drosophila is influenced by various genetic and environmental factors that also affect meiotic recombination. Thus, the amount of somatic crossing over in a given chromosome is controlled by the genetic contents of the chromosomal set (WEAVER 1960) and by the presence of various genes (STERN 1936; KAPLAN 1953). It may also be affected by changes in temperature ...

Human somatic mutation assays as biomarkers of carcinogenesis.

This paper describes four assays that detect somatic gene mutations in humans: the hypoxanthine-guanine phosphoribosyl transferase assay, the glycophorin A assay, the HLA-A assay, and the sickle cell hemoglobin assay. Somatic gene mutation can be considered a biomarker of carcinogenesis, and assays for somatic mutation may assist epidemiologists in studies that attempt to identify factors assoc...