Transformation of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum, and Escherichia coli with the C. diphtheriae plasmid pNG2.
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چکیده
منابع مشابه
Corynebacterium diphtheriae, Corynebacterium ulcerans and Corynebacterium pseudotuberculosis—General Aspects
V. A. de. C. Azevedo Departmento de Biologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil Abstract Corynebacterium diphtheriae, Corynebacterium ulcerans and Corynebacterium pseudotuberculosis are potentially diphtheria toxin-producing microorganisms related to different infectious processes involving both human and animal hosts. This chapte...
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Strains of Corynebacterium ulcerans and Corynebacterium pseudotuberculosis (Corynebacterium ovis) were examined for the production of diphtheria toxin. A majority of C. ulcerans strains (25 of 37) and 1 C. pseudotuberculosis strain (1 of 14) gave a positive Elek test for diphtheria toxin, and for all strains but 1, production of diphtheria toxin was inhibited at the same level of Fe2+ as was th...
متن کاملScreening for Corynebacterium diphtheriae.
A throat swab from a 9 year old girl with pharyngitis yielded a non-toxigenic strain of Corynebacterium diphtheriae var mitis and Streptococcus group G. C pseudodiphtheriticum was isolated from the throats of two of her four brothers. In each case the isolate was sent to the reference laboratory before full identification. The growth was found to be mixed for one brother; the other isolate bein...
متن کاملNeuraminidase of Corynebacterium diphtheriae.
Neuraminidase activity has been found in a variety of strains of Corynebacterium diphtheriae, both toxinogenic and nontoxinogenic. The enzyme has been shown to be intracellular, possibly associated with the cytoplasmic membrane. Toxinogenic strains of the diphtheria bacillus, grown under conditions unsuitable for maximal toxin production, produce neuraminidase, and the enzyme has been purified ...
متن کاملToxinogeny in Corynebacterium diphtheriae.
In 1951, Freeman made the remarkable discovery that nontoxinogenic Corynebacterium diphtheriae became toxinogenic2 after association with the lytic phage B, previously described by Toshach (1950). From lysates of nontoxinogenic, nonlysogenic C. diphtheriae, Freeman and Morse (1951) established, through the isolation of single cells, lysogenic strains which were toxinogenic. The lysogenic clones...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1987
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.84.14.4964