Two Approaches for CAL-B-Catalyzed Enantioselective Deacylation of a Set of α-Phenyl Ethyl Esters: Organic Solvent with Sodium Carbonate and Micro-aqueous Medium

Herein, we report an efficient enantioselective cleavage of the acyl- moiety a set α- phenyl ethyl esters with different chain-lengths catalyzed by lipase B from Candida antarctica (CAL-B) comparing two reactional approaches: anhydrous media sodium carbonates and micro-aqueous medium. The deacylation is performed in organic solvent, presence Na2CO3 first case, addition drop phosphate buffer solution pH 7 second. results show high efficiency carbonate for enzymatic resolution all that term reactivity (31% ≤ conv 50%) selectivity (E > 200). While, during hydrolysis media, conversion strongly affected length acyl-chain side, decreases = 50% 1-phenylethyl acetate 1a to 19% 1-phenyethyl dodecanoate 6a, this, even if remains 89). In both conditions, CAL-B shows enantioselectivities favor (R)-1-phenyl ethanol enantiomer (conv 45%, E 200) but modulated form size side.