Ty3 Integrase Is Required for Initiation of Reverse Transcription
نویسندگان
چکیده
منابع مشابه
Ty3 integrase is required for initiation of reverse transcription.
The integrase (IN) encoded by the Saccharomyces cerevisiae retrovirus-like element Ty3 has features found in retrovirus IN proteins including the catalytic triad, an amino-terminal zinc-binding motif, and a nuclear localization sequence. Mutations in the amino- and carboxyl-terminal domains of Ty3 IN cause reduced accumulation of full-length cDNA in the viruslike particles. We show that the red...
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متن کاملIntegrase mediates nuclear localization of Ty3.
Retroviruses in nondividing cells and yeast retrotransposons must transit the nuclear membrane in order for integration to occur. Mutations in a bipartite basic motif in the carboxyl-terminal domain of the Ty3 integrase (IN) protein were previously shown to block transposition at a step subsequent to 3'-end processing of Ty3 extrachromosomal DNA. In this work, the Ty3 IN was shown to be suffici...
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متن کاملProteolytic processing of Ty3 proteins is required for transposition.
Ty3 is a retroviruslike element found in Saccharomyces cerevisiae. It encodes GAG3 and GAG3-POL3 polyproteins which are processed into mature proteins found in the Ty3 viruslike particle. In this study, the region encoding a protease that is homologous to retroviral aspartyl proteases was identified and shown to be required for production of mature Ty3 proteins and transposition. The Ty3 protea...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 2002
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.76.6.2804-2816.2002