Ultrasensitive single-nucleotide polymorphism detection using target-recycled ligation, strand displacement and enzymatic amplification
نویسندگان
چکیده
منابع مشابه
Ultrasensitive single-nucleotide polymorphism detection using target-recycled ligation, strand displacement and enzymatic amplification† †Electronic supplementary information (ESI) available: The preparation, modification and magnetic retrieval of MNPs, optimisation of the Cap-MNP probe and experimental procedures and the SNP discrimination at low target abundance (5 fmol). See DOI: 10.1039/c3nr01010d Click here for additional data file.
a School of Chemistry and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom. Fax:+44-113-3436565; E-mail: [email protected] (YG) and [email protected] (DZ). b Section of Pathology and Tumour Biology, Leeds Institute of Molecular Medicine, Wellcome Trust Brenner Building, St James’s University Hospital, University of Leeds, Leeds LS9 7TF, United...
متن کاملA lateral flow biosensor for detection of single nucleotide polymorphism by circular strand displacement reaction.
A lateral flow biosensor for detection of single nucleotide polymorphism based on circular strand displacement reaction (CSDPR) has been developed. Taking advantage of high fidelity of T4 DNA ligase, signal amplification by CSDPR, and the optical properties of gold nanoparticles, this assay has reached a detection limit of 0.01 fM.
متن کاملHomogeneous real-time detection of single-nucleotide polymorphisms by strand displacement amplification on the BD ProbeTec ET system.
BACKGROUND The BD ProbeTec ET System is based on isothermal strand displacement amplification (SDA) of target nucleic acid coupled with homogeneous real-time detection using fluorescent probes. We have developed a novel, rapid method using this platform that incorporates a universal detection format for identification of single-nucleotide polymorphisms (SNPs) and other genotypic variations. M...
متن کاملDetection of single-nucleotide polymorphism.
Identification of polymorphism is the key for genetic mapping, diagnosis and marker-assisted selection. Restriction fragment-length polymorphisms (RFLPs) and short tandem repeats (STRs), also known as microsatellites, are commonly used genetic markers. Until discovery of STRs, RFLPs were used for genetic mapping, but bi-allelic polymorphism of RFLP limited its use as a marker. Although STRs are...
متن کاملUltrasensitive electrochemical DNA detection based on dual amplification of circular strand-displacement polymerase reaction and hybridization chain reaction.
We developed a novel electrochemical strategy for ultrasensitive DNA detection using a dual amplification strategy based on the circular strand-displacement polymerase reaction (CSDPR) and the hybridization chain reaction (HCR). In this assay, hybridization of hairpin-shaped capture DNA to target DNA resulted in a conformational change of the capture DNA with a concomitant exposure of its stem....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nanoscale
سال: 2013
ISSN: 2040-3364,2040-3372
DOI: 10.1039/c3nr01010d