Ultrashort Peptide Bioinks Support Automated Printing of Large-Scale Constructs Assuring Long-Term Survival of Printed Tissue Constructs

We report about rationally designed ultrashort peptide bioinks, overcoming severe limitations in current bioprinting procedures. Bioprinting is increasingly relevant tissue engineering, regenerative and personalized medicine due to its ability fabricate complex scaffolds through an automated deposition process. Printing stable large-scale constructs with high shape fidelity enabling long-term cell survival are major challenges that most existing bioinks unable solve. Additionally, they require chemical or UV-cross-linking for the structure-solidifying process which compromises encapsulated cells, resulting restricted structure complexity low viability. Using as ideal bodylike but synthetic material, we demonstrate instant solidifying cell-embedding printing via a sophisticated extrusion procedure under true physiological conditions at cost-effective bioink concentrations. Our printed chondrogenic differentiation of mesenchymal stem cells point strong potential fabrication.