Unbiased yeast screens identify cellular pathways affected in Niemann–Pick disease type C

Multiple Unbiased Prospective Screens Identify TRP Channels and Their Conserved Gating Elements

It is now clear that transient receptor potential (TRP) channels are sensors of temperature, mechanical force, noxious chemicals, and G protein – coupled receptor – mediated signal transduction. Sequence and topologi-cal analyses group TRPs with other tetrameric cation channels (Yu and Catterall, 2004). Each subunit bears extensive N-and C-terminal cytoplasmic domains and an S1-S2-S3-S4-S5-P-S6...

Small Molecule Screens to Identify Inhibitors of Infectious Disease

Overexpression screens identify conserved dosage chromosome instability genes in yeast and human cancer.

Somatic copy number amplification and gene overexpression are common features of many cancers. To determine the role of gene overexpression on chromosome instability (CIN), we performed genome-wide screens in the budding yeast for yeast genes that cause CIN when overexpressed, a phenotype we refer to as dosage CIN (dCIN), and identified 245 dCIN genes. This catalog of genes reveals human orthol...

Forward Genetic Screens in Zebrafish Identify Pre-mRNA-Processing Pathways Regulating Early T Cell Development

Lymphocytes represent basic components of vertebrate adaptive immune systems, suggesting the utility of non-mammalian models to define the molecular basis of their development and differentiation. Our forward genetic screens in zebrafish for recessive mutations affecting early T cell development revealed several major genetic pathways. The identification of lineage-specific transcription factor...

Vitamin D suppresses cellular pathways of diabetes complication in liver

Objective(s): The aim of this study was to investigate the effect of vitamin D on glucose metabolism, as well as the expression of five key genes involved in the development of diabetes complications in liver tissue of diabetic rats. Materials and Methods: Twenty-four male Sprague–Dawley rats were randomly divided into three groups (8 ra...