UNIVERSAL PRIMERS FOR DETECTION OF COMMON BACTERIAL PATHOGENS CAUSING PROSTHETIC JOINT INFECTION
نویسندگان
چکیده
منابع مشابه
Universal primers for detection of common bacterial pathogens causing prosthetic joint infection.
The diagnosis of low grade prosthetic joint infection is difficult and time consuming. Nested-PCR for universal bacterial DNA segments detection of "orthopaedic" bacteria was tested in a laboratory setting. This method is based on amplification of the 16S bacterial ribosomal RNA coding sequences. 11 species of the most frequent bacterial pathogens (Staphylococcus aureus, Staphylococcus epidermi...
متن کاملmolecular detection of common bacterial pathogens causing meningitis
background: the clinical diagnosis of meningitis is crucial, particularly in children. the early diagnosis and empiric antibiotic treatments have led to a reduction in morbidity and mortality rates. pcr and the enzymatic digestion of 16srdna fragment which is produced by universal primers led up fast and sensitive determination. the purpose of this study was to investigate a rapid method fo...
متن کاملuniversal primers used for detection of bacterial meningitis
background: acute bacterial meningitis is among serious infections of the central nervous system (cns). the early diagnosis and empiric antibiotic treatments have led to a reduction in morbidity and mortality rates. pcr and the enzymatic digestion of 16s rdna fragment following the pcr by universal primers led up fast and sensitive determination. the aims of the present study was to improve our...
متن کاملUse of PCR with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid.
We have designed a universal PCR capable of amplifying a portion of the 16S rRNA gene of eubacteria, including Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus pneumoniae, Enterococcus faecium, Enterococcus faecalis, Mycobacterium tuberculosis, Legionella pneumophila, Escherichia coli, Klebsiella pneumoniae, Serratia marcescens, ...
متن کاملIn vitro antibacterial activity of bioactive glass S53P4 on multiresistant pathogens causing osteomyelitis and prosthetic joint infection
BACKGROUND Conventional local treatment for medullary osteomyelitis (OM) includes insertion of antibiotic-loaded polymethylmethacrylate (PMMA) cement. Nevertheless, PMMA may delivery irregular concentration of antibiotic to surrounding tissue. We aimed to compare the in vitro antibacterial activity of Bioactive Glass (BAG) S53P4, which is a compound showing local antibacterial activity, to that...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biomedical Papers
سال: 2005
ISSN: 1213-8118,1804-7521
DOI: 10.5507/bp.2005.043