Use of GC Clamps in DHPLC Mutation Scanning
نویسندگان
چکیده
منابع مشابه
Efficient IDUA Gene Mutation Detection with Combined Use of dHPLC and Dried Blood Samples
Objectives. Development of a simple mutation directed method in order to allow lowering the cost of mutation testing using an easily obtainable biological material. Assessment of the feasibility of such method was tested using a GC-rich amplicon. Design and Methods. A method of denaturing high-performance liquid chromatography (dHPLC) was improved and implemented as a technique for the detectio...
متن کاملDNA Sequence Fragment Containing C to A Mutation as a Convenient Mutation Standard for DHPLC Analysis
Objective(s): Denaturing high performance liquid chromatography (DHPLC) is a high throughput approach for screening DNA sequence variations. To assess oven calibration, cartridge performance, buffer composition and stability, the WAVE Low and High Range Mutation Standards are employed to ensure reproducibility and accuracy of the chromatographic analysis. The purpose of this study was to provi...
متن کاملUse of DHPLC for rapid screening of recombinant clones.
Denaturing HPLC (DHPLC) is a relatively novel technique that has been mostly applied to molecular detection of pathological gene alterations (1,2). The method relies on the identification of heteroduplex molecules, due to mismatches between wild-type and mutant alleles, that are visualized in the form of altered chromatograms. Other nonconventional applications of DHPLC have also been reported,...
متن کاملDNA Sequence Fragment Containing C to A Mutation as a Convenient Mutation Standard for DHPLC Analysis
OBJECTIVE(S) Denaturing high performance liquid chromatography (DHPLC) is a high throughput approach for screening DNA sequence variations. To assess oven calibration, cartridge performance, buffer composition and stability, the WAVE Low and High Range Mutation Standards are employed to ensure reproducibility and accuracy of the chromatographic analysis. The purpose of this study was to provide...
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ژورنال
عنوان ژورنال: Clinical Medicine & Research
سال: 2003
ISSN: 1539-4182,1554-6179
DOI: 10.3121/cmr.1.2.111