Use of Nucleic Acid Amplification Testing for Diagnosis of Extragenital Sexually Transmitted Infections

Use of nucleic acid amplification testing for diagnosis of anorectal sexually transmitted infections.

Nucleic acid amplification testing (NAAT) has become the preferred method to detect Chlamydia trachomatis and Neisseria gonorrhoeae, but no commercial tests are cleared by the U.S. Food and Drug Administration for use with rectal swab samples. This study evaluated the performance of strand displacement amplification (SDA) and transcription-mediated amplification (TMA) to detect C. trachomatis a...

Sensitivity and Specificity of Nucleic Acid Sequence-Based Amplification Method for Diagnosis of Cutaneous Leishmaniasis

Abstract Background and Objective: Culture, microscopic method is a gold standard method for identification of Lishmania parasite. The use of Molecular methods such as RT- PCR compared to microscopic methods has a higher sensitivity and specificity however, it is not widely used due to its expensive equipment and the time requested. The use of nucleic acid sequence based amplification (NASBA) ...

Nucleic Acid amplification tests for diagnosis of Bordetella infections.

The global epidemiology of pertussis has recently been reviewed (12, 15). Bordetella pertussis continues to circulate even in populations where high vaccination coverage of infants and children is achieved (15, 23), because the protection after natural infection wanes after 10 to 15 years and protection after vaccination lasts for 6 to 10 years (15). A significant increase of pertussis cases wa...

Laboratory diagnosis of sexually transmitted infections.

Identification of sexually transmitted bacteria in tubo-ovarian abscesses through nucleic acid amplification.

Rodrigue Dessein, Géraldine Giraudet, Laure Marceau, Eric Kipnis, Sébastien Galichet, Jean-Philippe Lucot, Karine Faure Institut de Microbiologie, Laboratoire de Bactériologie Hygiène, Centre de Biologie Pathologie, CHRU de Lille, Service de Gynécologie-Obstétrique, Hôpital Jeanne de Flandre, CHRU de Lille, Service de Maladies Infectieuses, Hôpital Claude Huriez, CHRU de Lille, and Host-Pathoge...