Use of radioactive ethanolamine incorporation into phospholipids to assess in vitro antimalarial activity by the semiautomated microdilution technique
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چکیده
منابع مشابه
Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique.
A rapid, semiautomated microdilution method was developed for measuring the activity of potential antimalarial drugs against cultured intraerythrocytic asexual forms of the human malaria parasite Plasmodium falciparum. Microtitration plates were used to prepare serial dilutions of the compounds to be tested. Parasites, obtained from continuous stock cultures, were subcultured in these plates fo...
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1. Nerve cell bodies were isolated in bulk from cerebral cortices of 15 day-old rabbits after intrathecal injections of [(3)H]plamitate, [(3)H]oleate or [(3)H]arachidonate and [(14)C]glycerol. 2. Nuclear, microsomal and two mitochondrial fractions were isolated from homogenates of the radioactively labelled nerve cell bodies by using differential and discontinuous-gradient centrifugation. 3. Af...
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The phospholipid composition of human platelets and erythrocytes has been reported by several investigators (1-4) and the metabolism of phospholipids in human blood has been studied in some detail (5-9). Recent investigations of phospholipid metabolism of leukocytes have been reported (6, 10) but we are not aware of similar studies on platelets. In this laboratory, investigation of the biochemi...
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15 صفحه اولThe Incorporation of Radioactive Sulfur into Thiamine by Saccharomyces Cerevisiae.
The biosynthesis of thiamine has been believed to be accomplished with the initial formation of the pyrimidine and thiazole moieties by independent routes, followed by a final step in which pyrimidine and thiazole are jointed together to form thiamine. Recently, Nose (1-3), Brown (4-7), and Leder (8, 9) separated the enzyme system from cell-free extracts of bakers' yeast which is capable of syn...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1992
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.36.1.50