Using of Modern Genetic Methods to Detect Air-Borne Bacterial Genes Through Desert Storms in Iraq

Abstract Antibiotic resistance of pathogens is a serious issue that affects health. The airborne particle molecules such as (PM 2.5 , PM 10 ) spread by dust storms can increase the antibiotic in bacterial community. In this study air samples were collected and air-borne bacteria cultured on specific growth media portable sampler device (SAS). Sixty plates used through atmospheric which included three periods 24/5/2022,13/6/2022 3/7/2022. isolates was detected VITEK2. Bacterial whole DNA extracted genes against most resisted antibiotics pre-designed primers using gel electrophoresis method. results showed homogenous high concentrations both PM2.5 PM10 storm just like Saudi Arabia. greatest numbers collecting 45 60 (75%) E. coli 36 (60%) Staphylococcus epidermis 15 (25%) Sphingomonas paucimobilis total diagnosed morphological characteristics VITEK2 system. sensitivity test (AST) done VITIK2 multidrug resistant for epidermis, strains, mainly erythromycin tertacycline. genetic studies illustrated presence gene fragments ermA tetK within nucleic acid, are considered potent (ARGs genes). conclusion, towards elevated strongly relation with expression ARGs associated huge amounts particles occurred Iraq, very harmful human other living forms earth.