Using Superfolder Green Fluorescent Protein for Periplasmic Protein Localization Studies
نویسندگان
چکیده
منابع مشابه
Using superfolder green fluorescent protein for periplasmic protein localization studies.
Studies investigating the subcellular localization of periplasmic proteins have been hampered by problems with the export of green fluorescent protein (GFP). Here we show that a superfolding variant of GFP (sfGFP) is fluorescent following Sec-mediated transport and works best when the cotranslational branch of the pathway is employed.
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2011
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00315-11