Utilization of gradient front in liquid phase chromatography
نویسندگان
چکیده
منابع مشابه
High speed gradient elution reversed-phase liquid chromatography.
A major disadvantage of gradient elution in terms of speed results from the need to adequately re-equilibrate the column. This work distinguishes two states of re-equilibration: (1) run-to-run repeatability and (2) full equilibration. We find that excellent repeatability (+/-0.002 min in retention time) is achieved with at most 2 column volumes of re-equilibration whereas full equilibration can...
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The use of aqueous mixed micellar system consisting of sodium dodecyl sulfate (SDS) and polyoxyethylene (23) dodecanol (Brij-35) as the mobile phase in reversed-phase liquid chromatography was studied. A group of chlorophenols was used as the test mixture. Adding organic modifier to the system showed that the use of low concentrations of organic additives improves efficiency in SDS/Brij-35 mixe...
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The concept of limiting peak purity was applied to quantify the degree of completion of the separation capability of a chromatographic system using multi-linear gradients. The objective was to check whether the complexity of a gradient program deserves be increased to enhance resolution by inserting more linear segments, or on the contrary, no significant improvements can be expected under more...
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A new mathematical treatment concerning the gradient elution in reversed-phase liquid chromatography when the volume fraction psi of an organic modifier in the water-organic mobile phase varies linearly with time is presented. The experimental ln k versus psi curve, where k is the retention factor under isocratic conditions in a binary mobile phase, is subdivided into a finite number of linear ...
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In previous studies stationary phase optimized selectivity liquid chromatography (SOSLC) has been already proved as a promising technique to optimize the selectivity of a given separation [1, 2]. By combination of different stationary phases, SOSLC offers excellent possibilities for method development under both isocratic and linear gradient conditions. However, the so far available commercial ...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 1968
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.17.1416