UV spectrophotometric method development and validation for estimation of ketoconazole in bulk and pharmaceutical dosage form

: For the determination of ketoconazole in bulk and pharmaceutical dosage form accordance with ICH guidelines, a new, economical, precise, sensitive, linear, accurate, quick UV-Spectrophotometric approach has been developed Methylene chloride. The UV-visible spectrum Ketoconazole was examined to determine its maximum absorption wavelength that is λ max at 255.2 nm. Linearity, accuracy, precision robustness tested for this procedure. found have absorbance regression coefficient concentration range 5–25 µg/ml. Ketoconazole. LOD LOQ were determined be 0.0225 0.75 µg/ml, respectively. procedure successfully used on commercial formulation, outcomes good agreement label claims. Depending results, given method can applied Pharmaceutical form.