Validated Bio-Analytical Method for Cinacalcet Concentrations in Rat Plasma by Reversed Phase-High Performance Liquid Chromatography- Photodiode Array Detector Method

The goal of this work was to create and verify a simple reversed phase-high performance liquid chromatography-photodiode array detector-based method for quantifying cinacalcet in rat plasma. Six albino Wistar rats were given orally by oral gavage. Blood obtained at regular intervals using retro-orbital vein puncture processed get Cinacalcet extracted from plasma 70:30 v/v ethanol:dichloromethane combination, with an 82.80 %-104.08 % recovery rate. detector technique used determine concentrations. mobile phase acetonitrile:0.1 M dipotassium phosphate buffer (pH 6.8) (50:50 v/v) flow rate 1.0 ml/min. 241.0 nm as the detecting wavelength. method's linearity proven correlation coefficient (R2) more than 0.9996 linear range 1 μg/ml 24 cinacalcet. lower upper limits quantification found be respectively. At cinacalcets retention time, no interfering peaks seen. This approach is straightforward effective way pharmacokinetic parameters measuring levels.