Validation and Application of Liquid Chromatography Coupled with Tandem Mass Spectrometry Method for the Analysis of Glyphosate, Aminomethylphosphonic Acid (AMPA), and Glufosinate in Soil

A method was developed to determine glyphosate, aminomethylphosphonic acid (AMPA), and glufosinate in soil. The worldwide use of this herbicide agricultural activities, its known negative effects on both the environment health, have generated interest establishment methodologies for determination several matrices at trace level. development analytical methods AMPA, is challenging due present amphoteric properties, high solubility water, low molecular weight, affinity ions presents soil, lack chromophore groups structure, making quantification difficult. proposed exhibits a linear range from 5.0 600 µg/kg with limits detection 1.37, 0.69 1.22 μg/kg, 4.11, 2.08, 3.66 μg/kg glufosinate, respectively, adequate repeatability reproducibility (coefficients variation <8.0% recovery percentages between 93.56% 99.10%). matrix effect calculated each analyte, proving be good alternative these contaminants. described applied 46 soil samples collected crop fields Hidalgo, Mexico, concentrations varying not detected 4.358 (for AMPA).