Validity of Low Copy Number Typing and Applications to Forensic Science
نویسندگان
چکیده
منابع مشابه
Validity of low copy number typing and applications to forensic science.
Low copy number (LCN) typing, particularly for current short tandem repeat (STR) typing, refers to the analysis of any sample that contains less than 200 pg of template DNA. Generally, LCN typing simply can be defined as the analysis of any DNA sample where the results are below the stochastic threshold for reliable interpretation. There are a number of methodologies to increase sensitivity of ...
متن کاملUse of Low Copy Number (lcn) Dna in Forensic Inference
Development of a low copy number DNA profiling method has allowed the detection of very low levels of DNA. However, issues concerning the transfer and persistence of LCN DNA have required further research to be carried out; this has included studies of individuals’ tendency to deposit and transfer DNA onto surfaces they have touched. Additionally, the effect on DNA of some of the enhancement tr...
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We reviewed the current methodologies used for human DNA quantitation in forensic and ancient DNA studies, including sensitive hybridization methods based on the detection of nuclear alpha-satellite repetitive DNA regions or more recently developed fluorogenic real-time polymerase chain reaction (PCR) designs for the detection of both nuclear andmitochondrial DNA regions. Special emphasis has b...
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Low copy number (LCN) DNA profiling is a technique sensitive enough to analyze just a few cells. When this kind of analysis is carried out, special considerations are needed to interpret the results. In particular, it is important to consider the implications of allele dropout and the possibility of contamination from a laboratory source. A rationale for interpreting LCN DNA is described.
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Producing molecular imprinting-based materials has received increasing attention due to recognition selectivity, stability, cast effectiveness, and ease of production in various forms for a wide range of applications. The molecular imprinting technique has a variety of applications in the areas of the food industry, environmental monitoring, and medicine for diverse purposes like sample pretrea...
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ژورنال
عنوان ژورنال: Croatian Medical Journal
سال: 2009
ISSN: 0353-9504,1332-8166
DOI: 10.3325/cmj.2009.50.207