Variant Specific Cleavage of the Na + ‐Ca 2+ Exchanger NCX3 During Excitotoxicity

Cleavage of the Plasma Membrane Na+/Ca2+ Exchanger in Excitotoxicity

In brain ischemia, gating of postsynaptic glutamate receptors and other membrane channels triggers intracellular Ca2+ overload and cell death. In excitotoxic settings, the initial Ca2+ influx through glutamate receptors is followed by a second uncontrolled Ca2+ increase that leads to neuronal demise. Here we report that the major plasma membrane Ca2+ extruding system, the Na+/Ca2+ exchanger (NC...

Function and Regulation of the Na -Ca Exchanger NCX3 Splice Variants in Brain and Skeletal Muscle*

Gender influences [Ca(2+)](i) during metabolic inhibition in myocytes overexpressing the Na(+)-Ca(2+) exchanger.

BACKGROUND The Na(+)-Ca(2+) exchanger (NCX) may contribute to Ca(2+) overload and injury in ischemic cardiomyocytes. Recently, NCX overexpression was reported to increase ischemia/reperfusion injury in male and oophorectomized female but not in female mice. We therefore measured the effects of gender and estrogen on [Ca(2+)](i) and [Na(+)](i) during metabolic inhibition (MI) in myocytes from wi...

Actin-dependent regulation of the cardiac Na(+)/Ca(2+) exchanger.

In the present study, the bovine cardiac Na(+)/Ca(2+) exchanger (NCX1.1) was expressed in Chinese hamster ovary cells. The surface distribution of the exchanger protein, externally tagged with the hemagglutinin (HA) epitope, was associated with underlying actin filaments in regions of cell-to-cell contact and also along stress fibers. After we treated cells with cytochalasin D, NCX1.1 protein c...

Increased Na(+)-Ca(2+) exchanger in the failing heart.

Heart failure (HF) is a significant problem, affecting more than 2 million people in the United States alone.1 When severe, it is associated with a 50% 2-year mortality rate attributable to either contractile dysfunction or sudden death from ventricular tachycardia and ventricular fibrillation.1 The underlying molecular mechanisms have not yet been defined, but there is considerable evidence fr...