Vibrational Spectroscopy for Imaging Single Microbial Cells in Complex Biological Samples

Vibrational Spectroscopy for Imaging Single Microbial Cells in Complex Biological Samples

Vibrational spectroscopy is increasingly used for the rapid and non-destructive imaging of environmental and medical samples. Both Raman and Fourier-transform infrared (FT-IR) imaging have been applied to obtain detailed information on the chemical composition of biological materials, ranging from single microbial cells to tissues. Due to its compatibility with methods such as stable isotope la...

Vibrational spectroscopy and imaging.

Single Photon Counting X-Ray Micro-Imaging of Biological Samples

Biological Imaging Spectroscopy

Improved detectors, new electro-optical devices and vastly improved computational power for data analysis have fueled the recent interest in combining biology and spectroscopy. This review article will cover three aspects of biomedical spectroscopy. (1 ) Data acquisition: what instruments are available for acquiring an image cube and what are the performance trade-offs involved in choosing one ...

Investigating microbial (micro)colony heterogeneity by vibrational spectroscopy.

Fourier transform infrared and Raman microspectroscopy are currently being developed as new methods for the rapid identification of clinically relevant microorganisms. These methods involve measuring spectra from microcolonies which have been cultured for as little as 6 h, followed by the nonsubjective identification of microorganisms through the use of multivariate statistical analyses. To exa...