Visualizing sphingolipid biosynthesis in cells

The N-glycosylation defect of cwh8∆ yeast cells causes a distinct defect in sphingolipid biosynthesis

CWH8/YGR036c of Saccharomyces cerevisiae has been identified as a dolichylpyrophosphate phosphatase that removes a phosphate from the dolichylpyrophosphate (Dol-PP) generated by the oligosaccharyltransferase while it adds N-glycans to nascent glycoproteins in the ER. Lack of CWH8 was proposed to interrupt the so called dolichol cycle by trapping dolichol in the form of Dol-PP in the ER lumen. I...

Organization and functions of sphingolipid biosynthesis in yeast.

Sphingolipids are important signalling molecules and thus it is very important to understand how they are generated. Sphingolipid biosynthesis shows a conserved compartmentalization in eukaryotic cells. Their synthesis begins in the endoplasmic reticulum and is completed in the Golgi apparatus. We now know quite a bit about the topology of the reactions in the pathway, but certain critical step...

The N-glycosylation defect of cwh8Delta yeast cells causes a distinct defect in sphingolipid biosynthesis.

CWH8/YGR036c of Saccharomyces cerevisiae has been identified as a dolichylpyrophosphate (Dol-PP) phosphatase that removes a phosphate from the Dol-PP generated by the oligosaccharyltransferase (OST), while it adds N-glycans to nascent glycoproteins in the endoplasmic reticulum (ER). Lack of CWH8 was proposed to interrupt the so called dolichol (Dol) cycle by trapping Dol in the form of Dol-PP i...

Total synthesis of the sphingolipid biosynthesis inhibitor fumonisin B(1).

The first total synthesis of the sphingolipid biosynthesis inhibitor fumonisin B(1) has been achieved. This convergent synthesis utilizes oxonia Cope rearrangements to prepare two key homoallylic alcohols, which are then functionalized to the primary components A and B for cross-coupling. Other highlights of our approach include a new and efficient synthesis of the diprotected tricarballylic ac...

De novo sphingolipid biosynthesis: a necessary, but dangerous, pathway.