White spot syndrome virus VP28 specific double-stranded RNA provides protection through a highly focused siRNA population
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چکیده
منابع مشابه
White spot syndrome virus VP24 interacts with VP28 and is involved in virus infection.
White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. Herein, the characterization of VP24, a major structural protein of WSSV, is described. When purified virions were subjected to Nonidet P-40 treatment to separate the envelopes from the nucleocapsids, VP24 was found to be present exclusively in the envelope fraction. Triton X-114 extraction ...
متن کاملPmRab7 is a VP28-binding protein involved in white spot syndrome virus infection in shrimp.
Our aim was to isolate and characterize white spot syndrome virus (WSSV)-binding proteins from shrimp. After a blot of shrimp hemocyte membrane proteins was overlaid with a recombinant WSSV envelope protein (rVP28), the reactive bands on the blot were detected using anti-VP28 antibody. Among three membrane-associated molecules identified by liquid chromatography-tandem mass spectrometry, there ...
متن کاملRecombinant VP28 Produced by Cell-Free Technique Confers Protection in Kuruma Shrimp (Marsupenaeus japonicus) Against White Spot Syndrome Virus
Shrimp is one of the most important species in aquaculture. During the last two decades, shrimp culture has been threatened worldwide by viral diseases. A highly pathogenic white spot syndrome virus (WSSV) is responsible for huge economic losses in the shrimp culture industry, and no effective treatment is currently available for it (Rajesh Kumar et al., 2008). Cumulative mortalities in infecte...
متن کاملOral Vaccination of Baculovirus-Expressed VP28 Displays Enhanced Protection against White Spot Syndrome Virus in Penaeus monodon
White Spot Syndrome Virus (WSSV) is an infectious pathogen of shrimp and other crustaceans, and neither effective vaccines nor adequate treatments are currently available. WSSV is an enveloped dsDNA virus, and one of its major envelope proteins, VP28, plays a pivotal role in WSSV infection. In an attempt to develop a vaccine against WSSV, we inserted the VP28 gene into a baculovirus vector tail...
متن کاملThe protection of CpG ODNs and Yarrowia lipolytica harboring VP28 for shrimp Litopenaeus vannamei against White spot syndrome virus infection
The white spot syndrome is one of the most serious disease which has caused high mortalities and huge economic losses to shrimp culture. In the present study, the oral administrations with CpG ODNs and Yarrowia lipolytica harboring VP28 (rVP28-yl) as dietary supplement for shrimp Litopenaeus vannamei were conducted to evaluate their protective effects against WSSV. After feeding for 15 days, th...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/s41598-017-01181-w