Whole-Genome Sequencing for Bacterial Strain Typing Using the iSeq100 Platform

Application of whole-genome sequencing for bacterial strain typing in molecular epidemiology.

Nosocomial infections pose a significant threat to patient health; however, the gold standard laboratory method for determining bacterial relatedness (pulsed-field gel electrophoresis [PFGE]) remains essentially unchanged 20 years after its introduction. Here, we explored bacterial whole-genome sequencing (WGS) as an alternative approach for molecular strain typing. We compared WGS to PFGE for ...

Molecular Methods for Bacterial Strain Typing

ABSTRACT        Typing of bacteria is an important part of epidemiological studies on nosocomial infections. Bacterial identification methods have dramatically improved in recent years, which is mainly due to advancements in the field of molecular biotechnology. In many cases, molecular techniques have replaced phenotypic typing methods. Currently, a wide r...

A Bacterial Analysis Platform: An Integrated System for Analysing Bacterial Whole Genome Sequencing Data for Clinical Diagnostics and Surveillance

Recent advances in whole genome sequencing have made the technology available for routine use in microbiological laboratories. However, a major obstacle for using this technology is the availability of simple and automatic bioinformatics tools. Based on previously published and already available web-based tools we developed a single pipeline for batch uploading of whole genome sequencing data f...

molecular methods for bacterial strain typing

abstract        typing of bacteria is an important part of epidemiological studies on nosocomial infections. bacterial identification methods have dramatically improved in recent years, which is mainly due to advancements in the field of molecular biotechnology. in many cases, molecular techniques have replaced phenotypic typing methods. currently, a wide range of bacterial typing techniques is...

Identification and typing of Salmonella for public health surveillance using whole genome sequencing