Whole-transcriptome analysis reveals virulence-specific pathogen−host interactions at the placenta in bovine neosporosis
نویسندگان
چکیده
Research on bovine neosporosis has achieved relevant milestones, but the mechanisms underlying occurrence of foetal death or protection against remain unclear. In a recent study, placentas from heifers challenged with high-virulence isolate Nc-Spain7 exhibited focal necrosis and inflammatory infiltrates as soon 10 days post-infection (dpi), although parasite detection was minimal. These lesions were more frequent at 20 dpi, coinciding higher rates in some animals. contrast, such not observed animals infected low-virulence Nc-Spain1H, where detected only placenta one animal dpi. This work aimed to study which are triggered (caruncles cotyledons) these pregnant early stages infection (10 dpi) through whole-transcriptome analysis. caruncles, provoked strong proinflammatory response effect isolate, IL-6/JAK/STAT3 signalling TNF-alpha via NF-κB pathways down-regulated. Interestingly, expression E2F target genes, related restraining response, At pronounced gene signatures detectable being intense carrying dead fetuses. However, continued without activating response. cotyledons, similar that caruncles; however, induced mild signals Finally, deconvolutional analysis both placentome tissues revealed markedly fraction activated natural killers, M1 macrophages CD8+ T cells for isolate. Therefore, our transcriptomic supports hypothesis an immune probably by multiplication could be key contributor abortion. Further studies required determine effectors govern distinct interactions high- isolates host, help elucidate molecular processes pathogenesis cattle.
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ژورنال
عنوان ژورنال: Frontiers in Immunology
سال: 2023
ISSN: ['1664-3224']
DOI: https://doi.org/10.3389/fimmu.2023.1198609