Yeast telomeric sequences function as chromosomal anchorage points invivo
نویسندگان
چکیده
منابع مشابه
Yeast telomeric sequences function as chromosomal anchorage points in vivo.
Site-specific recombination in Saccharomyces cerevisiae was used to generate non-replicative DNA rings containing yeast telomeric sequences. In topoisomerase mutants expressing Escherichia coli topoisomerase I, the rings adopted a novel DNA topology consistent with the ability of yeast telomeric DNA to block or retard the axial rotation of DNA. DNA fragments bearing portions of the terminal rep...
متن کاملExpansion of Interstitial Telomeric Sequences in Yeast.
Telomeric repeats located within chromosomes are called interstitial telomeric sequences (ITSs). They are polymorphic in length and are likely hotspots for initiation of chromosomal rearrangements that have been linked to human disease. Using our S. cerevisiae system to study repeat-mediated genome instability, we have previously shown that yeast telomeric (Ytel) repeats induce various gross ch...
متن کاملChromosomal aberrations involving telomeres and interstitial telomeric sequences.
Telomeres are specialised nucleoproteic complexes localised at the physical ends of linear eukaryotic chromosomes that maintain their stability and integrity. In vertebrate chromosomes, the DNA component of telomeres is constituted by (TTAGGG)n repeats, which can be localised at the terminal regions of chromosomes (true telomeres) or at intrachromosomal sites (interstitial telomeric sequences o...
متن کاملGenome rearrangements caused by interstitial telomeric sequences in yeast.
Interstitial telomeric sequences (ITSs) are present in many eukaryotic genomes and are linked to genome instabilities and disease in humans. The mechanisms responsible for ITS-mediated genome instability are not understood in molecular detail. Here, we use a model Saccharomyces cerevisiae system to characterize genome instability mediated by yeast telomeric (Ytel) repeats embedded within an int...
متن کاملThe association of yeast Ku with subtelomeric core X sequences prevents recombination involving telomeric sequences
The yKu protein of Saccharomyces cerevisiae is important for genome stability by repressing recombination involving telomeric sequences. The mechanism of this repression is not known but silent heterochromatin such as HML, HMR and telomeres are compartmentalized at the nuclear periphery and yKu is proposed to interact with these regions and play a role in telomeric silencing and tethering. We h...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1997
ISSN: 1460-2075
DOI: 10.1093/emboj/16.3.523