Yin Yang 1 Protein Negatively Regulates High-Density Lipoprotein Receptor Gene Transcription by Disrupting Binding of Sterol Regulatory Element Binding Protein to the Sterol Regulatory Element

Transcription factor sterol regulatory element binding protein 2 regulates scavenger receptor Cla-1 gene expression.

OBJECTIVE The human scavenger receptor class B type I (Cla-1) plays a key role in cellular cholesterol movement in facilitating transport of cholesterol between cells and lipoproteins. Indirect evidence has suggested that Cla-1 gene expression is under the feedback control of cellular cholesterol content. To define the molecular mechanisms underlying such putative regulation, we evaluated wheth...

[Sterol regulatory element binding protein (SREBP)].

Sterol Regulatory Element-Binding Protein 1 Is Negatively Modulated by Protein Kinase A Phosphorylation

Sterol regulatory element binding protein (SREBP) 1a and 1c are key transcription factors that regulate lipid biosynthesis in cells. We identified that Ser-338 located at the N-terminus of SREBP1a is a protein kinase A (PKA) phosphorylation site in vitro and in HepG2 cells. PKA phosphorylation of this site attenuated DNA occupancy, as revealed by ChIP assay, and the ensuing transactivation. In ...

Androgens stimulate lipogenic gene expression in prostate cancer cells by activation of the sterol regulatory element-binding protein cleavage activating protein/sterol regulatory element-binding protein pathway.

Using two independent prostate cancer cell lines (LNCaP and MDA-PCa-2a), we demonstrate that coordinated stimulation of lipogenic gene expression by androgens is a common phenomenon in androgen-responsive prostate tumor lines and involves activation of the sterol regulatory element-binding protein (SREBP) pathway. We show 1) that in both cell lines, androgens stimulate the expression of fatty a...

Multiple mechanisms, independent of sterol regulatory element binding proteins, regulate low density lipoprotein gene transcription.

Transcription of the LDL receptor gene is markedly enhanced in the Jurkat T cell line by stimulation with the combination of the phorbol ester phorbol 12-myristate 13-acetate (PMA) and the protein synthesis inhibitor cycloheximide (CHX). The DNA sequences necessary for this response were identified by analysis of Jurkat T cells permanently transfected with reporter gene expression vectors conta...