Yolk proteins from nematodes, chickens, and frogs bind strongly and preferentially to left-handed Z-DNA.

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Yolk proteins from nematodes, chickens, and frogs bind strongly and preferentially to left-handed Z-DNA.

Yolk proteins purified from the nematode Caenorhabditis elegans, from the frog Xenopus laevis, and from chicken eggs all have the unexpected property of binding strongly and preferentially to a left-handed Z-DNA probe, brominated poly(dG-dC). We estimate that the nematode proteins bind to Z-DNA with an association constant of at least 10(4) (M-1) and that this association constant is at least 4...

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The structure of Z-DNA, currently accepted as a model for all left-handed DNAs, fails to provide convincing explanations for at least four well established properties of left-handed DNA polymers in solution. However, the major discrepancies between theory and experiment are resolved by the structure presently proposed for Z[WC]-DNA, a new left-handed, zig-zag double helix with Watson-Crick-type...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1988

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)37390-3