rapid detection of different serovares of salmonella entrica by multiplex pcr


a karami

r ranjbar

z ahmadi

z safiri


background: typhoid fever is still one of the serious public health problems in many geographic areas and is endemic in most countries. aim of current study was to evaluate a shortened time –multiplex pcr for rapid detection of different sal monella enterica serovars. methods: the pcr primers for three target genes tyv, prt and inva were subjected for amplification by pcr. by using sim ple dna extraction method, rapid pcr cycles and rapid electrophoresis procedure with simple and very cheap buffer were utilized in 200 to 300 volts for 15 minutes to separate the pcr products. results: the results showed that all reference and clinical isolates of s. enterica were accurately identified by this as say with no cross reaction with other enterobacterial strains tested. detection limit of the reaction was to be fewer than 10-1 colony forming unit. conclusion: these data indicate that the optimized rapid cycle multiplex pcr is a potentially valuable tool for rapid diagno sis of s. enterica using a conventional thermal cycler. this method reduced the reaction time of pcr from 3.5 h to less than 1 h.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Selective Amplification of prt, tyv and invA Genes by Multiplex PCR for Rapid Detection of Salmonella typhi

A multiplex PCR-based assay was developed for detection of Salmonella typhi and identification of other salmonella serotypes. Three primer-sets were selected from different genomic sequences, malo2-F/malo2-Ra primers from invasion gene, Parat-s/Parat-as as well as tyv-s/tyv-as primers from O-antigen gene cluster of the genus Salmonella. This method differentiated Salmonella spp., based on size ...

متن کامل

rapid and specific detection of salmonella typhimurium by pcr-elisa

objectives: salmonella typhimurium is important food-borne pathogen responsible for gastroenteritis. in this work a polymerase chain reaction based enzyme linked immunosorbent assay (pcr-elisa) was developed to identify salmonella typhimurium. materials and methods: the rfb gene which is responsible for biosynthesis of the salmonella o-antigenic lipopolysaccharide was selected as the target s...

متن کامل

Comparing Rapid and Specific Detection of Brucella in Clinical Samples by PCR-ELISA and Multiplex-PCR Method

Background: Rapid diagnosis and differentiation of Brucella is of high importance due to the side effects of antibiotics for the treatment of brucellosis. This study aimed to identify and compare PCR-ELISA as a more accurate diagnositc test with other common molecular and serological tests. Methods: ...

متن کامل

Rapid detection of carbapenemase genes by multiplex real-time PCR.

OBJECTIVES To develop a single multiplex real-time PCR assay to detect six different genetic types of carbapenemases already identified in Enterobacteriaceae (KPC, GES, NDM, IMP, VIM and OXA-48). METHODS A total of 58 bacterial isolates were tested. Thirty were previously characterized as resistant to carbapenems and documented by PCR and sequencing analysis to carry the following genes: bla(...

متن کامل

Rapid detection of methicillin-resistant staphylococci by multiplex PCR.

A multiplex PCR was developed to detect the coagulase gene (coa; pathognomic of Staphylococcus aureus) and the mecA gene (characteristically encoding for methicillin resistance in staphylococci) in a single, rapid test. Suitable primers for the gene targets and an internal, amplification control were incorporated into a multiplex PCR assay, which was then optimized on a capillary air thermal cy...

متن کامل

Rapid and Concurrent Detection of Listeria Species by Multiplex Pcr

Listeria sps are ubiquitous in nature. Infection due to L. monocytogenes causes illness in animals and humans worldwide. Aim of the present study was to standardize a multiplex PCR for the identification and differentiation of important Listeria species, in particular, Listeria monocytogenes and to detect toxigenic potential of L. monocytogenes. Employing primers for truncated regions of seven ...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
iranian journal of public health

جلد ۳۶، شماره ۲، صفحات ۳۸-۴۲

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023