Tumor-Infiltrating Lymphocytes Derived From Select B-Cell Lymphomas Secrete Granulocyte-Macrophage Colony-Stimulating Factor and Tumor Necrosis Factor-a in Response to Autologous Tumor Stimulation
نویسنده
چکیده
Tumor infiltrating lymphocytes (TIL) were cultured from 17 B-cell lymphoma specimens derived from patients with predominantly low-grade malignancies. Specimens included 15 lymph-node biopsies, 1 malignant pleural effusion, and PBL from 1 patient with circulating lymphoma cells. The phenotypic and proliferative characteristics of TIL cultured in interleukin-2 (IL-2) were studied, as well as cytolysis and cytokine secretion in response to autologous tumor. Flow cytometry of fresh tumor suspensions showed that 50% of cells (median) were malignant B cells and 36% were infiltrating T lymphocytes. After culture for approximately 1 month, TIL were 75% f 8% CD3' (mean f SEM), 47% f 8% CD4+ and 35% f 7% CD8'. TIL proliferation was modest in most cases: the median maximum expansion was 32-fold in 25 days. Lysis of autologous tu-
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تاریخ انتشار 2003