Plasmid transformation and replica filter plating of Acholeplasma laidlawii.

Expression of foreign genes and selection of promoter sequences in Acholeplasma laidlawii.

The stable maintenance and expression of foreign genes in mollicutes (mycoplasmas) have been difficult to achieve due to the lack of suitable vectors. In this paper we show for the first time that a replicating vector can been used to express foreign genes other than antibiotic resistance genes in Acholeplasma laidlawii. Plasmids derived from the lactococcal vector pNZ18 could introduce and mai...

Mycoplasmatales virus-laidlawii 2, a new virus isolated from Acholeplasma laidlawii.

Effects of synthetic cecropin analogs on in vitro growth of Acholeplasma laidlawii.

Four synthetic peptides (Peptidyl MIMs; Demeter Biotechnologies, Inc.) were evaluated for their in vitro activity against Acholeplasma laidlawii. Fifty percent effective concentration values ranged from 1 to 15 microM. Three of these compounds are more lethal than cecropin B against A. laidlawii.

Genome Sequences of Acholeplasma laidlawii Strains with Increased Resistance to Tetracycline and Melittin

Acholeplasma laidlawii is a well-suited model for studying the molecular basis for adapting mollicutes to environmental conditions. Here, we present the whole-genome sequences of two strains of A. laidlawii with increased resistance to tetracycline and melittin.

Lysis of Acholeplasma laidlawii by antibodies and complement.

Membranes of Acholeplasma laidlawii were used to determine the membrane components involved in immune lysis and to eventually detect enzymatic changes in the membrane components during this reaction. In a previous publication we reported that A. laidlawii can be killed by antibody and complement and that antibodies to membrane proteins are by far more effective than antibodies to membrane lipid...