Preparation of unfixed undemineralized bone sections: the Bright bone cryostat.

نویسندگان

  • A D Pearse
  • D L Gardner
چکیده

A bone cryostat is described with which cold sections of unfixed, undemineralized calcified tissue can be prepared. The bone cryostat is suitable for the preparation of the sections required for the histochemical and immunofluorescent study of bone, for electron microprobe analysis, and for bone and joint autoradiography with soluble compounds.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Demonstration of light chain monotypia in B cell non-Hodgkin's lymphomas using unfixed freeze-dried and formalin-fixed trypsinised paraffin sections.

Immunohistological light chain analysis was carried out in 55 patients with non-Hodgkin's lymphoma of B cell origin. Unfixed freeze-dried paraffin sections were used to detect surface Ig and formaldehyde-fixed trypsinised sections to detect cytoplasmic Ig. Cytoplasmic Ig was seen inconstantly in freeze-dried paraffin sections. There was complete agreement with regard to the type of light chain ...

متن کامل

Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues

Monoclonal antibodies were produced against the recently described short chain cartilage collagen (type X collagen), and one (AC9) was extensively characterized and used for immunohistochemical localization studies on chick tissues. By competition enzyme-linked immunosorbent assay, antibody AC9 was observed to bind to an epitope within the helical domain of type X collagen and did not react wit...

متن کامل

Electron microscopy of undecalcified human bone.

An alternative approach for the electron microscopical examination of undecalcified human bone was investigated. The method required bone to be chilled to -70 degrees C, sectioned at 10 microns in a special bone cryostat, and these sections to be fixed and embedded for ultrathin sectioning. Good preservation of bone cells was seen. The advantages of this method are that it allows numerous parti...

متن کامل

Localization of GFP in frozen sections from unfixed mouse tissues: immobilization of a highly soluble marker protein by formaldehyde vapor.

Green fluorescent protein (GFP) and its variants, such as enhanced GFP (EGFP), have been introduced into mammalian cells by transgenes, e.g., to distinguish donor from host cells after transplantation. Free GFP is extremely soluble and leaks out from liquid-covered cryostat sections so that fixation of whole organs before sectioning has been mandatory. This precludes the analysis of serial sect...

متن کامل

Enzyme histochemical analysis on cryostat sections of human bone marrow.

A recently developed procedure, that has been shown to be suitable for detailed immunohistological analysis, has been used to prepare cryostat sections of bone marrow to investigate whether enzyme-histochemical techniques are also feasible on such material. A selected group of enzymes, some of which are inhibited or destroyed in paraffin- or plastic-embedded samples, have been demonstrated. The...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of clinical pathology

دوره 25 1  شماره 

صفحات  -

تاریخ انتشار 1972