Simultaneous Estimation of Irbesartan and Hydrochlorothiazide in Combined Pharmaceutical Dosage Form by UV Spectroscopy using Multi- component Mode of Analysis

A simple, accurate, precise and economical procedure for simultaneous estimation of irbesartan and hydrochlorothiazide in combined tablet dosage form has been developed utilizing concept of standard addition. The method is based upon determination of irbesartan at 234nm and hydrochlorothiazide at 272nm, in aqueous ethanol (30%v/v). Different analytical performance parameters such as linearity, precision, accuracy and ruggedness were determined according to ICH guidelines. Irbesartan and hydrochlorothiazide at their respective λmax shows linearity in the concentration range of 10-26μg/mL and 5-13μg/mL respectively. The method was validated statistically. The results of analysis formulation given as percentage of label claim were found to be 99.32±0.631 and 100.15±0.762 for irbesartan and hydrochlorothiazide respectively. Therefore, the proposed methods can be used for the routine analysis of both drugs in quality control laboratories.