Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase.
نویسندگان
چکیده
The avian sarcoma virus transforming gene product pp60src has been partially purified by using ion exchange or immunoaffinity chromatography. These preparations contain a cyclic AMP-independent protein kinase activity capable of transferring radiolabel from [gamma-32P]ATP to immune rabbit IgG, casein, and the heavy chain purified from normal rabbit IgG. The casein kinase activity is specifically inhibited by anti-pp60src IgG. Comparison of the pp60src-protein kinase isolated from cells transformed by a wild-type ASV or by an ASV temperature-sensitive transformation mutant revealed that the latter product had a half-life of 3 min at 41 degrees C whereas that of the wild-type product was 20 min.
منابع مشابه
Avian sarcoma virus UR2 encodes a transforming protein which is associated with a unique protein kinase activity.
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متن کاملUninfected vertebrate cells contain a protein that is closely related to the product of the avian sarcoma virus transforming gene (src).
Neoplastic transformation of cell by avian sarcoma virus is mediated by a single viral gene (src), which encodes a phosphoprotein (pp60src) with the enzymatic activity of a protein kinase. The DNAs of vertebrate species contain a highly conserved homologue of src that is also represented in the polysomal RNA of uninfected cells and, hence, may specify a normal cellular protein. We have used ant...
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 76 12 شماره
صفحات -
تاریخ انتشار 1979