In Vitro Studies on a Microfluidic Sensor with Embedded Obstacles Using New Antibacterial Synthetic Compounds (1-TDPPO) Mixed Prop-2-en-1-one with Difluoro Phenyl

نویسندگان

  • Chang Hyun Roh
  • Jaewoong Lee
  • Mayank Kinger
  • Chankyu Kang
چکیده

This paper describes the use of an analytical microfluidic sensor for accelerating chemo-repellent response and strong anti-bacterial 1-(Thien-2-yl)-3-(2, 6-difluoro phenyl) prop-2-en-1-one (1-TDPPO). The chemically-synthesized antimicrobial agent, which included prop-2-en-1-one and difluoro phenyl groups, was moving through an optically transparent polydimethylsiloxane (PDMS) microfluidic sensor with circular obstacles arranged evenly. The response, growth and distribution of fluorescent labeling Pseudomonas aeruginosa PAO1 against the antimicrobial agent were monitored by confocal laser scanning microscope (CLSM). The microfluidic sensor along with 1-TDPPOin this study exhibits the following advantages: (i) Real-time chemo-repellent responses of cell dynamics; (ii) Rapid eradication of biofilm by embedded obstacles and powerful antibacterial agents, which significantly reduce the response time compared to classical methods; (iii) Minimal consumption of cells and antimicrobial agents; and (iv) Simplifying the process of the normalization of the fluorescence intensity and monitoring of biofilm by captured images and datasets.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Synthesis and study of antibacterial activity of some 1-phenyl-3-aryl-5-(4-(2-ethanoloxy) phenyl)-1H-pyrazoles

A series of 1-phenyl-3-aryl-5-(4-(2-ethanoloxy) phenyl)-1H-pyrazoles were synthesized from chalcones and studied for their in vitro antibacterial activity. Chalcones i.e.,1-aryl-3-(4hydroxyphenyl) prop-2-en-1-ones, 1 on reaction with phenyl hydrazine yielded the corresponding 1-phenyl-3-aryl-5-(4-hydroxyphenyl)-1H-pyrazoles 2 which on further reaction with 3-chloroethanol furnished the title co...

متن کامل

Synthesis and Antibacterial Activity of Some 1-Phenyl-3-aryl-5-(4-(3-propanoloxy) phenyl)-1H-pyrazoles

A series of 1-phenyl-3-aryl-5-(4-(3-propanoloxy) phenyl)-1H-pyrazoles were synthesized from chalcones and studied for their in vitro antibacterial activity. Chalcones i.e.,1-aryl-3-(4hydroxyphenyl) prop-2-en-1-ones (1) on reaction with phenyl hydrazine in presence of acetic acid and few drops of HCl yielded the corresponding 1-phenyl-3-aryl-5-(4-hydroxyphenyl)-1H-pyrazoles (2) which on further ...

متن کامل

سنتز و ارزیابی برون‌تنی اثرات ضد‌قارچی و ضد‌باکتریایی مشتقات 2و3-دی‌هیدروکینازولینونی

 Introduction: By development of bacterial drug-resistance to traditional antibacterial drugs and fungal  infections, the need to design, synthesis, and use of new and effective antibacterial and antifungal drugs is increased. Quinazolinone derivatives are heterocyclic compounds with nitrogen atoms that have pharmacological properties such as anti-bacterial, anti-tumor, anti-inflammatory and an...

متن کامل

(E)-3-(2-Chloro­phen­yl)-1-(4,4′′-difluoro-5′-meth­oxy-1,1′:3′,1′′-terphenyl-4′-yl)prop-2-en-1-one

The title compound, C(28)H(19)ClF(2)O(2), is a polysubstituted terphenyl derivative bearing a Michael system in which the C=C double bond has an E conformation. In the crystal, C-H⋯Cl and C-H⋯O contacts connect the mol-ecules into layers lying perpendicular to the a axis. The shortest inter-centroid distance between symmetry-related 4-fluoro-phenyl groups is 3.7547 (16) Å.

متن کامل

(E)-1-(4,4′′-Difluoro-5′-meth­oxy-1,1′:3′,1′′-terphenyl-4′-yl)-3-(4-methyl­phen­yl)prop-2-en-1-one

In the meta-terphenyl fragment of the title mol-ecule, C(29)H(22)F(2)O(2), the two fluoro-phenyl rings are twisted from the central benzene ring by 46.72 (6) and 41.70 (6)°, respectively. In the crystal, weak C-H⋯O and C-H⋯F hydrogen bonds link the mol-ecules into layers parallel to the ab plane. The crystal packing exhibits π-π inter-actions, the shortest distance between the centroids of arom...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 17  شماره 

صفحات  -

تاریخ انتشار 2017