Vitrification Technique – New Possibilities for Male Gamete Low-Temperature Storage

The method is cheap and quick. Due to lowering the temperature of solution's glass transition the permeable cryoprotectants prevents the actual freezing of solution and allows to maintain its some flexibility in a glassy phase. Vitrification without permeable cryoprotectants allows to avoid the cryoprotectants toxicity and osmotic stress; the damage of plasmatic and mitochondrial membrane during equilibration with cryoprotectants; protects of plasmatic and mitochondrial membrane against lipid peroxidation and formation of reactive oxygen species and DNA damage.