TAFII 105 mediates activation of anti - apoptotic genes by NF - κ B
نویسنده
چکیده
The transcription factor NF-κB is important for expression of genes involved in immune responses, viral infections, cytokine signaling and stress. In addition NF-κB plays a crucial role in protecting cells from TNFα-induced apoptotic stimuli, presumably by activating anti-apoptotic genes. Here we report that the substoichiometric TFIID subunit TAFII105 is essential for activation of anti-apoptotic genes in response to TNFα , serving as a transcriptional coactivator for NF-κB. The putative coactivator domain of TAFII105 interacts with the activation domain of the p65/RelA member of the NF-κB family, and further stimulates p65induced transcription in human 293 cells. Moreover, inhibition of TAFII105 activity by overexpression of a dominant negative mutant of TAFII105 decreased NF-κB transcriptional activity and severely reduced cell survival in response to TNF-α. Similarly, expression of anti-sense TAFII105 RNA sensitized the cells to TNFα cytotoxicity. These results suggest that TAFII105 is involved in activation of anti-apoptotic genes by NF-κB.
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تاریخ انتشار 2013