Development of a robust protocol for gene expression analysis using formalin-fixed, paraffin-embedded liver transplant biopsy specimens

Expression of Survivin in the Formalin-Fixed, Paraffin-embedded Specimens of Human Osteosarcoma May Be Used as a Diagnostic Marker

Osteosarcoma is the most frequent malignant bone tumor with a peak incidence in the second and third decades of life. Diagnosis of patients with osteosarcoma is limited to clinico-pathological parameters whereas molecular markers of tumor initiation and promotion have not yet been identified. We aimed to study the expression of the anti-apoptotic survivin gene in osteosarcoma specimens. A total...

Detection of Survivin Gene Expression in Formalin-Fixed Paraffin-Embedded Tissues of Human Osteosarcoma: Its Potential Usefulness in Diagnosis and Prognosis of Bone Tumors

Osteosarcoma is a relatively uncommon malignancy however, it is the most common form of primary malignant bone tumors in human. Diagnosis and prognosis of patients with osteosarcoma is limited to clinico-radiopathological parameters, whereas molecular markers of tumor aggression have been poorly identified. Survivin, an inhibitor of apoptosis (IAP), is unique for its expression in human tumors ...

Diagnostic value of direct immunofluorescence of mucosal and skin biopsy specimens on formalin-fixed paraffin-embedded biopsy specimens compared to specimens placed in normal saline as the gold standard method in the diagnosis of a group of autoimmun

Background and Aim: Direct immunofluorescence (DIF) is represented as a gold standard method in diagnosis of autoimmune blistering dermatoses. Normal saline, liquid nitrogen and michel’ solution are a widely accepted media for preserving biopsy samples of skin or mucosa before DIF examination. Occasionally clinicians put the biopsy specimen taken for DIF in formalin 10%, occasionally clinicians...

Molecular classification of mature aggressive B-cell lymphoma using digital multiplexed gene expression on formalin-fixed paraffin-embedded biopsy specimens.

Sonification as a means of enhancing the detection of gene expression levels from formalin-fixed, paraffin-embedded biopsies.

We describe a novel approach for the isolation and quantification of mRNA from archival specimens by using oligo(dT)25 paramagnetic beads and the reverse transcription polymerase chain reaction (RT-PCR). A preliminary study is conducted to evaluate experimental conditions and the effects of varied periods of sonification on the yield of mRNA isolated from formalin-fixed, paraffin-embedded human...