The Triton X-100 and High Salt Resistant Residue of Saccharomyces cerevisiae Nuclear Membranes, II

Nuclear Membrane, Insoluble Membrane Residue, G 1 Phase of Cell Cycle, a Mating Pheromone, Anti-Lamine Antibodies The Triton X-100 and High salt resistant residue of the nuclear envelope of Saccharomyces cerevisiae cells arrested in the G 1 phase of cell cycle with a mating pheromone was isolated. It showed the same electrophoretic protein pattern as the insoluble residue isolated from log-phase cells. Examination of nitrocellulose blots of nuclear protein fractions with antiserum raised against a vertebrate lamin failed to show any immunological cross-reaction.