Detection of cfxA and cfxA2, the -Lactamase Genes of Prevotella spp., in Clinical Samples from Dentoalveolar Infection by Real-Time PCR
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Detection of cfxA and cfxA2, the -Lactamase Genes of Prevotella spp., in Clinical Samples from Dentoalveolar Infection by Real-Time PCR Kaori Iwahara, Tomoari Kuriyama,* Satoshi Shimura, David W. Williams, Maki Yanagisawa, Kiyomasa Nakagawa, and Tadahiro Karasawa Department of Oral and Maxillofacial Surgery, Kanazawa University Graduate School of Medical Science, Department of Oral and Maxillofacial Surgery, Kanazawa University Hospital, and Department of Clinical Laboratory Science, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan, and Department of Oral Surgery, Medicine and Pathology, School of Dentistry, Cardiff University, Cardiff, United Kingdom
منابع مشابه
Detection of cfxA and cfxA2, the beta-lactamase genes of Prevotella spp., in clinical samples from dentoalveolar infection by real-time PCR.
While most bacteria involved in dentoalveolar infection are highly susceptible to penicillin, some Prevotella strains exhibit resistance to this agent through the production of beta-lactamase. The production of beta-lactamase by Prevotella spp. is in turn associated with the expression of the genes cfxA and cfxA2. The aim of the present study was to determine the prevalence of cfxA and cfxA2 in...
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Purpose The aim of this study was to identify β-lactamase-producing oral anaerobic bacteria and screen them for the presence of cfxA and BlaTEM genes that are responsible for β-lactamase production and resistance to β-lactam antibiotics. Material and Methods Periodontal pocket debris samples were collected from 48 patients with chronic periodontitis and anaerobically cultured on blood agar plat...
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A prospective analysis on β-lactam resistance mechanisms and β-lactamase prevalence was conducted on Prevotella intermedia and Prevotella nigrescens recovered from patients with chronic periodontitis and peritonsillar abscesses. Both phenotypic and genotypic methods were performed to characterize the β-lactamases, their coding genes and their genetic contexts. Overall, β-lactamase production wa...
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OBJECTIVES To investigate mechanisms of reduced susceptibility to commonly used antibiotics in Prevotella cultured from patients with cystic fibrosis (CF), patients with invasive infection and healthy control subjects and to determine whether genotype can be used to predict phenotypic resistance. METHODS The susceptibility of 157 Prevotella isolates to seven antibiotics was compared, with det...
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A beta-lactamase gene (cfxA3, 966 bp) was isolated from a beta-lactam-resistant Capnocytophaga ochracea clinical isolate and amplified using primers from the cfxA gene of Bacteroides vulgatus. The MICs of third-generation cephalosporins were much higher than those of the transconjugant Escherichia coli strain. The deduced protein sequence, by comparison with CfxA2 of Prevotella intermedia, had ...
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تاریخ انتشار 2005