Regulation of Tcra / Tcrd Locus Conformation during Thymocyte Development
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چکیده
Regulation of Tcra/Tcrd locus conformation during thymocyte development by Han-Yu Department of Immunology Duke University Date:_______________________ Approved: ___________________________ Michael Krangel, Supervisor ___________________________ Yuan Zhuang, Chair ___________________________ Shyam Unniraman ___________________________ Qijing Li ___________________________ Kenneth Kreuzer ___________________________ Laura Rusche An abstract of a dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Department of Immunology in the Graduate School of Duke University 2012 Copyright by Han-Yu Shih 2012
منابع مشابه
Distinct contracted conformations of the Tcra/Tcrd locus during Tcra and Tcrd recombination
Studies have suggested that antigen receptor loci adopt contracted conformations to promote long-distance interactions between gene segments during V(D)J recombination. The Tcra/Tcrd locus is unique because it undergoes highly divergent Tcrd and Tcra recombination programs in CD4(-)CD8(-) double negative (DN) and CD4(+)CD8(+) double positive (DP) thymocytes, respectively. Using three-dimensiona...
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Antigen receptor locus V(D)J recombination requires interactions between widely separated variable (V), diversity (D), and joining (J) gene segments, but the mechanisms that generate these interactions are not well understood. Here we assessed mechanisms that direct developmental stage-specific long-distance interactions at the Tcra/Tcrd locus. The Tcra/Tcrd locus recombines Tcrd gene segments ...
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Murine Tcra and Tcrd gene segments are organized into a single genetic locus (Tcra/Tcrd locus) that undergoes V(D)J recombi-nation in CD4 2 CD8 2 double-negative (DN) thymocytes to assemble Tcrd genes and in CD4 + CD8 + double-positive thymocytes to assemble Tcra genes. Recombination events are regulated by two developmental stage-specific enhancers, E d and E a. Effects of E a on Trca/Tcrd loc...
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TCRb, d and c chain genes are assembled and expressed in double-negative thymocytes prior to ab or cd T cell lineage commitment. Thus, cells committed to the ab T cell lineage can possess completely assembled TCRd and/or TCRc chain genes. However, these genes are not expressed. TCRc chain gene expression may be silenced through the activity of a cis-acting silencer element. In the TCRa/d locus,...
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Murine Tcrd and Tcra gene segments reside in a single genetic locus and undergo recombination in CD4- CD8- (double negative [DN]) and CD4+ CD8+ (double positive [DP]) thymocytes, respectively. TcraTcrd locus variable gene segments are subject to complex regulation. Only a small subset of approximately 100 variable gene segments contributes substantially to the adult TCRdelta repertoire. Moreove...
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تاریخ انتشار 2012